Gene description for CD81
Gene name	CD81 molecule
Gene symbol	CD81
Other names/aliases	CVID6 S5.7 TAPA1 TSPAN28
Species	Homo sapiens
Experiment description of studies that identified CD81 in B cells
1 Experiment ID 11 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 12519789    Organism Homo sapiens Experiment description Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation. Authors Wubbolts R, Leckie RS, Veenhuizen PT, Schwarzmann G, Mös W, Hoernschemeyer J, Slot JW, Geuze HJ, Stoorvogel W Journal name JBC Publication year 2003 Sample B cells Sample name RN (HLA-DR15+) Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [MALDI TOF] Mass spectrometry [QTOF] Western blotting Thin layer chromatography High performance liquid chromatography EV-TRACK -
2 Experiment ID 16 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 9685355    Organism Homo sapiens Experiment description Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes. Authors Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ Journal name JBC Publication year 1998 Sample B cells Sample name RN (HLA-DR15+) Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.12-1.18 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Immunoelectron microscopy EV-TRACK -
3 Experiment ID 47 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method FACS PubMed ID 17641064    Organism Homo sapiens Experiment description Exosomes with immune modulatory features are present in human breast milk. Authors Admyre C, Johansson SM, Qazi KR, FiléJJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S Journal name JIMMU Publication year 2007 Sample B cells Sample name B cells Isolation/purification methods Differential centrifugation Filtration Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study FACS EV-TRACK -
4 Experiment ID 79 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 20458337    Organism Homo sapiens Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1 Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. Journal name ICB Publication year 2010 Sample B cells Sample name RN (HLA-DR15) Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [FT-ICR] Western blotting EV-TRACK EV100035: EV-METRIC:44%
5 Experiment ID 80 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 20458337    Organism Homo sapiens Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2 Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. Journal name ICB Publication year 2010 Sample B cells Sample name RN (HLA-DR15) Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [FT-ICR] Western blotting EV-TRACK EV100035: EV-METRIC:44%
6 Experiment ID 81 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 20458337    Organism Homo sapiens Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3 Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. Journal name ICB Publication year 2010 Sample B cells Sample name RN (HLA-DR15) Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [FT-ICR] Western blotting EV-TRACK EV100035: EV-METRIC:44%
7 Experiment ID 116 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Mass spectrometry PubMed ID 21505438    Organism Homo sapiens Experiment description Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells. Authors Mittelbrunn M, Gutiéez-Váuez C, Villarroya-Beltri C, Gonzáz S, Sáhez-Cabo F, Gonzáz MÁ Bernad A, Sáhez-Madrid F. Journal name NATCOM Publication year 2011 Sample B cells Sample name Raji Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Mass spectrometry Microarray EV-TRACK EV110049: EV-METRIC:11%
8 Experiment ID 271 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Immunoelectron microscopy PubMed ID 10903724    Organism Homo sapiens Experiment description Follicular dendritic cells carry MHC class II-expressing microvesicles at their surface Authors Denzer K, van Eijk M, Kleijmeer MJ, Jakobson E, de Groot C, Geuze HJ Journal name J Immunol Publication year 2000 Sample B cells Sample name RN Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Immunoelectron microscopy EV-TRACK -